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Multiplex IHC and IF Content

Mosaic Laboratories offers multiplex brightfield immunohistochemistry and multiplex immunofluorescence assays to expand the power of your biomarker analyses. Multiplex immunohistochemistry has played a critical role in immuno-oncology therapy clinical trials to evaluate the density and nature of immune cells infiltration and the spatial distribution of cells. Performing multiplex immunohistochemistry has many potential benefits and applications in the field of drug target evaluation and patient screening, including:
1) determination and quantification of immunoreactivity co-localization;
2) evaluation of spatial relationships between immunoreactive cell types;
3) signal transduction pathway activation studies;
4) reduction in tissue depletion; and
5) decreased reagent utilization. Multiplex assays may be designed using multiplex chromogenic detection combined with multispectral imaging or immunofluorescence.

Sample Assays:

  • AKR1C3 + CD34 + CD117
  • Aurora A + pAurora A
  • Aurora A + pAurora A + pHH3
  • Aurora A + pHH3
  • B7-H3 + CD23
  • B7-H3 + DR5 + CD138
  • B7-H3 + DR5 + CD34 / CD117
  • CD117 + IGFBP-2
  • CD137 + PD-1
  • CD138 + CD38
  • CD138 + cMYC
  • CD138 + pHH3
  • CD138 + pERK 20G11
  • CD3 + CD4
  • CD3 + CD8
  • CD3 + FoxP3
  • CD3 + PD-1
  • CD3 + Ki67 + CD4
  • CD3 + CD4 + FoxP3
  • CD31 + Cleaved Caspase-3 + Ki-67
  • CD34 + CD117 (cKIT) + AURKA (20q13)/20q11
  • CD34 + CD31
  • CD34 + Cleaved Caspase-3
  • CD34 + pFLT3
  • CD34 + pHH3
  • CD37 + CD20
  • CD37 + CD38
  • CD38 + CD138 + Total FOXO 3a
  • CD38 + CD138 + Cleaved Caspase-3
  • CD38 + CD138 + Ki-67
  • CD38 + CD138 + pAKT
  • CD38 + CD138 + pAKT T308
  • CD38 + CD138 + pFOXO
  • CD38 + CD138 + pGSK3β
  • CD38 + CD138 + pPRAS40
  • CD38 + CD138 + Total AKT
  • CD38 + CD138 + Total FOXO 3a
  • CD38 + CD138 + Total GSK3β
  • CD38 + CD138 + Total PRAS40
  • CD4 + Ki-67
  • CD4 + CD3 + FoxP3
  • CD4 + CD8 + FoxP3
  • CD45R0 + CD31
  • CD68 + CD163
  • CD68 + PD-L1
  • CD8 + Ki-67
  • CD8 + PD-L1
  • CD8 + FoxP3
  • CD8 + FoxP3 + PD-1
  • CD68 + PD-L1 +CD3
  • CDC2 + pCDC2
  • CIAP1 + XIAP + CD34 + CD117
  • cIAP2 + CD117 + CD34
  • E-Cadherin + FSP-1 + NG2
  • E-Cadherin + NG2
  • ERK1 + ERK2
  • FSP-1 + NG2
  • GFAP + CH24H
  • IGF1R + InsR
  • Ki-67 + Cleaved caspase-3
  • Lag3 + FoxP3 + CD8
  • Lag3 + MHCII + CD68
  • MHCI + MHCII
  • p70S6K T389 + Total p70SK
  • p70S6K T421/S424 + Total p70S6K
  • pAKT + Total AKT
  • PD-1 + CD45RO + CD8
  • pERK 20G11 + pAKT (MOS140)
  • pERK + Total ERK
  • pHH3 + Ki-67
  • pHH3 + Cleaved Caspase-3 + Ki-67
  • PLK + Ki-67
  • pPRAS40 + Total PRAS40
  • Total BAD + pBAD
  • Total p27 + pp27

Custom Assay Development and Validation is Available upon Request

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